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Registro Completo |
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
27/01/2021 |
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Data da última atualização: |
27/01/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOUTO, B. de M.; ARAÚJO, A. C. B. de; HAMANN, P. R. V.; BASTOS, A. de R.; CUNHA, I. de S.; PEIXOTO, J.; KRUGER, R. H.; NORONHA, E. F.; QUIRINO, B. F. |
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Afiliação: |
BETULIA DE MORAIS SOUTO, CNPAE; Ana Carolina Bitencourt de Araújo; Pedro Ricardo Vieira Hamann, Universidade de Brasília; Andrêssa de Rezende Bastos; Isabel de Souza Cunha, Universidade Católica de Brasília; Julianna Peixoto, Universidade de Brasília; Ricardo Henrique Kruger, Universidade de Brasília; Eliane Ferreira Noronha, Universidade de Brasília; BETANIA FERRAZ QUIRINO, CNPAE. |
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Título: |
Functional screening of a Caatinga goat (Capra hircus) rumen metagenomic library reveals a novel GH3 beta-xylosidase. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
PLoS ONE, v. 16, n. 1, e024511, 2021. |
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DOI: |
https://doi.org/10.1371/journal.pone.0245118 |
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Idioma: |
Inglês |
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Conteúdo: |
Functional screening of metagenomic libraries is an effective approach for identification of novel enzymes. A Caatinga biome goat rumen metagenomic library was screened using esculin as a substrate, and a gene from an unknown bacterium encoding a novel GH3 enzyme, BGL11, was identified. None of the BGL11 closely related genes have been previously characterized. Recombinant BGL11 was obtained and kinetically characterized. Substrate specificity of the purified protein was assessed using seven synthetic aryl substrates. Activity towards nitrophenyl-beta-D-glucopyranoside (pNPG), 4-nitrophenyl- beta -D-xylopyranoside (pNPX) and 4-nitrophenyl- beta -D-cellobioside (pNPC) suggested that BGL11 is a multifunctional enzyme with beta-glucosidase, beta-xylosidase, and cellobiohydrolase activities. However, further testing with five natural substrates revealed that, although BGL11 has multiple substrate specificity, it is most active towards xylobiose. Thus, in its native goat rumen environment, BGL11 most likely functions as an extracellular beta-xylosidase acting on hemicellulose. Biochemical characterization of BGL11 showed an optimal pH of 5.6, and an optimal temperature of 50°C. Enzyme stability, an important parameter for industrial application, was also investigated. At 40°C purified BGL11 remained active for more than 15 hours without reduction in activity, and at 50°C, after 7 hours of incubation, BGL11 remained 60% active. In contrast to BLG11, most beta-xylosidases kinetically studied belong to the GH43 family and have been characterized only using synthetic substrates. In industry, beta-xylosidases can be used for plant biomass deconstruction, and the released sugars can be fermented into valuable bio-products, ranging from the biofuel ethanol to the sugar substitute xylitol. MenosFunctional screening of metagenomic libraries is an effective approach for identification of novel enzymes. A Caatinga biome goat rumen metagenomic library was screened using esculin as a substrate, and a gene from an unknown bacterium encoding a novel GH3 enzyme, BGL11, was identified. None of the BGL11 closely related genes have been previously characterized. Recombinant BGL11 was obtained and kinetically characterized. Substrate specificity of the purified protein was assessed using seven synthetic aryl substrates. Activity towards nitrophenyl-beta-D-glucopyranoside (pNPG), 4-nitrophenyl- beta -D-xylopyranoside (pNPX) and 4-nitrophenyl- beta -D-cellobioside (pNPC) suggested that BGL11 is a multifunctional enzyme with beta-glucosidase, beta-xylosidase, and cellobiohydrolase activities. However, further testing with five natural substrates revealed that, although BGL11 has multiple substrate specificity, it is most active towards xylobiose. Thus, in its native goat rumen environment, BGL11 most likely functions as an extracellular beta-xylosidase acting on hemicellulose. Biochemical characterization of BGL11 showed an optimal pH of 5.6, and an optimal temperature of 50°C. Enzyme stability, an important parameter for industrial application, was also investigated. At 40°C purified BGL11 remained active for more than 15 hours without reduction in activity, and at 50°C, after 7 hours of incubation, BGL11 remained 60% active. In contrast to BLG11, most beta-xylosidases kinetical... Mostrar Tudo |
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Thesagro: |
Clonagem; Enzima; Peptídeo; Programa de Computador. |
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Thesaurus Nal: |
Cloning (animals); Computer software; Enzymes; Metagenomics; Prevotella; Screening; Signal peptide. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/220708/1/Functional-screening-of-a-Caatinga-goat-2021.pdf
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Marc: |
LEADER 02852naa a2200361 a 4500
001 2129624
005 2021-01-27
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1371/journal.pone.0245118$2DOI
100 1 $aSOUTO, B. de M.
245 $aFunctional screening of a Caatinga goat (Capra hircus) rumen metagenomic library reveals a novel GH3 beta-xylosidase.$h[electronic resource]
260 $c2021
520 $aFunctional screening of metagenomic libraries is an effective approach for identification of novel enzymes. A Caatinga biome goat rumen metagenomic library was screened using esculin as a substrate, and a gene from an unknown bacterium encoding a novel GH3 enzyme, BGL11, was identified. None of the BGL11 closely related genes have been previously characterized. Recombinant BGL11 was obtained and kinetically characterized. Substrate specificity of the purified protein was assessed using seven synthetic aryl substrates. Activity towards nitrophenyl-beta-D-glucopyranoside (pNPG), 4-nitrophenyl- beta -D-xylopyranoside (pNPX) and 4-nitrophenyl- beta -D-cellobioside (pNPC) suggested that BGL11 is a multifunctional enzyme with beta-glucosidase, beta-xylosidase, and cellobiohydrolase activities. However, further testing with five natural substrates revealed that, although BGL11 has multiple substrate specificity, it is most active towards xylobiose. Thus, in its native goat rumen environment, BGL11 most likely functions as an extracellular beta-xylosidase acting on hemicellulose. Biochemical characterization of BGL11 showed an optimal pH of 5.6, and an optimal temperature of 50°C. Enzyme stability, an important parameter for industrial application, was also investigated. At 40°C purified BGL11 remained active for more than 15 hours without reduction in activity, and at 50°C, after 7 hours of incubation, BGL11 remained 60% active. In contrast to BLG11, most beta-xylosidases kinetically studied belong to the GH43 family and have been characterized only using synthetic substrates. In industry, beta-xylosidases can be used for plant biomass deconstruction, and the released sugars can be fermented into valuable bio-products, ranging from the biofuel ethanol to the sugar substitute xylitol.
650 $aCloning (animals)
650 $aComputer software
650 $aEnzymes
650 $aMetagenomics
650 $aPrevotella
650 $aScreening
650 $aSignal peptide
650 $aClonagem
650 $aEnzima
650 $aPeptídeo
650 $aPrograma de Computador
700 1 $aARAÚJO, A. C. B. de
700 1 $aHAMANN, P. R. V.
700 1 $aBASTOS, A. de R.
700 1 $aCUNHA, I. de S.
700 1 $aPEIXOTO, J.
700 1 $aKRUGER, R. H.
700 1 $aNORONHA, E. F.
700 1 $aQUIRINO, B. F.
773 $tPLoS ONE$gv. 16, n. 1, e024511, 2021.
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Esconder MarcMostrar Marc Completo |
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Registro original: |
Embrapa Agroenergia (CNPAE) |
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Biblioteca |
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| Registros recuperados : 44 | |
| 3. |  | ALVES, W. F.; ITO, E. N.; MARCONCINI, J. M.; ORLANDI, M. O.; MALMONGE, J. A.; MATTOSO, L. H. C. Characterization of nanocomposites of polyurethane based on castor oil and cellulose nanofibers. In: INTERNATIONAL CONFERENCE ON NANOSTRUCTURED MATERIALS -NANO 2008, 9., 2008, Rio de Janeiro. Abstracts... Rio de Janeiro: Centro Brasileiro de Pesquisas Físicas, 2008. p. 302.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
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| 4. | | CORDEIRO, E. M. S.; NUNES, Y. L.; MATTOS, A. L. A.; ROSA, M. de F.; SOUZA FILHO, M. de S. M. de; ITO, E. N. Polymer biocomposites and nanobiocomposites obtained from mango seeds. Macromolecular Symposia, v. 344, n. 1, p. 39-54, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
| Biblioteca(s): Embrapa Agroindústria Tropical. |
|  |
| 5. | | CORRADINI, E.; ITO, E. N.; MARCONCINI, J. M.; RIOS, C. T.; AGNELLI, J. A. M.; MATTOSO, L. H. C. Interfacial behavior of composites of recycled poly(ethylene terephthalate) and sugarcane bagasse fiber. Polymer testing, Essex, v. 28, n. 2, p. 183-187, 2009.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Instrumentação. |
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| 6. | | CORRADINI, E.; ITO, E. N.; MARCONCINI, J. M.; RIOS, C. T.; AGNELLI, J. A. M.; MATTOSO, L. H. C. Interfacial behaviour of composites of recycled poly(ethylene terephthalate) and sugar cane bagase fibers. In: INTERNATIONAL MACROMOLECULAR COLLOQUIUM - IMC, 11.; INTERNATIONAL SYMPOSIUM ON NATURAL POLYMERS AND COMPOSITES - ISNaPol, 6., 2007, Gramado, RS. Natural polymers and composites. Anais... [São Carlos: ABPol, 2007]. não paginado. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Instrumentação. |
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| 7. | | CORRADINI, E.; ITO, E. N.; MARCONCINI, J. M.; RIOS, C. T.; AGNELLI, J. A.; MATTOSO, L. H. C. Interfacial behaviour of composites of recycled poly(ethylene terephthalate) and sugar cane bagase fibers. In: INTERNATIONAL MACROMOLECULAR COLLOQUIUM - IMC, 11.; INTERNATIONAL SYMPOSIUM ON NATURAL POLYMERS AND COMPOSITES - ISNaPol, 6., 2007, Gramado, RS. Natural polymers and composites. Program and proceedings... [São Carlos: ABPol, 2007]. Oral 02. Não paginado.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
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| 8. | | CRUZ, M. R. DA; PEREIRA, L. M.; MORAIS, J. P. S.; BRÍGIDA, A. I. S.; ITO, E. N.; ROSA, M. de F. Obtenção de nanocelulose de línter utilizando rotas alternativas de hidrólise ácida. In: WORKSHOP DA REDE DE NANOTECNOLOGIA APLICADA AO AGRONEGÓCIO 2012, 6., 2012, Fortaleza. São Carlos: Embrapa Instrumentação; Fortaleza: Embrapa Agroindústria Tropical, 2012. p. 234-236| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Algodão. |
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| 12. | | ITO, E. N.; MARCONCINI, J. M.; PESSOA, J. D. C.; RIOS, C. T.; MATTOSO, L. H. C.; HAGE JUNIOR, E. Caracterização de compósitos de poliolefinas recicladas com fibras de açaí. In: CONGRESSO BRASILEIRO DE POLÍMEROS, 9., 2007, Campina Grande. Anais... [São Carlos: ABPol, 2007.]. não paginado. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Instrumentação. |
|  |
| 13. | | ITO, E. N.; MARCONCINI, J. M.; PESSOA, J. D.; MATTOSO, L. H. C.; RIOS, C. T.; HAGE JUNIOR, E. Caracterização de compósitos de poliolefinas recicladas com fibras de açaí. In: Congresso Brasileiro de Polímeros, 9., 2007, Campina Grande, PB. Resumos... [São Carlos: ABPol, 2007.]. p. 87. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
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| 14. | | ITO, E. N.; PEREIRA, L. M.; MARCONCINI, J. M. Study of dispersion and compatibilization of sugarcane bagasse fibers (SF) embedded in matrix of poly(ethylene terephthalate) recycled (PETrec) during processing In: INTERNATIONAL CONFERENCE ON FOOD AND AGRICULTURE APPLICATIONS OF NANOTECHNOLOGIES - NanoAgri, 2010, São Pedro, SP. [Anais…] São Pedro: Aptor Software, 2010. Editors: Caue Ribeiro, Odílio Benedito Garrido de Assis, Luiz Henrique Capparelli Mattoso, Sérgio Mascarenhas.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
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| 16. | | ITO, E. N.; ROSA, M. F.; MARCONCINI, J. M. Development of composites using low density polyethylene (LDPE) and coconut fibers (CF) In: INTERNATIONAL CONFERENCE ON FOOD AND AGRICULTURE APPLICATIONS OF NANOTECHNOLOGIES - NanoAgri, 2010, São Pedro, SP. [Anais…] São Pedro: Aptor Software, 2010. Editors: Caue Ribeiro, Odílio Benedito Garrido de Assis, Luiz Henrique Capparelli Mattoso, Sérgio Mascarenhas.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
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| 17. | | ITO, E. N.; SILVA, W. T. L.; MARCONCINI, J. M.; RIBEIRO, C.; MAGALHÃES, W. L. E.; HAGE JUNIOR, E.; MATTOSO, L. H. C. Effect of processing on the properties of poly (Methyl methacrylate) / silica nanocomposites. In: ANNUAL MEETING [OF] THE POLYMER PROCESSING SOCIETY, 23., 2007, Salvador. Program and book of abstracts. São Carlos: Polymer Processing & Reology Center DEMa/UFSCAR, 2007. PPS-23.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Florestas; Embrapa Instrumentação. |
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| 18. | | ITO, E. N.; SILVA, W. T. L.; MARCONCINI, J. M.; RIBEIRO, C.; MAGALHÃES, W. L. E.; MATTOSO, L. H. C.; HAGE JUNIOR, E. Effect of processing on the properties of poly (Methyl methacrylate) / silica nanocomposites. In: ANNUAL MEETING [OF] THE POLYMER PROCESSING SOCIETY - PPS, 23., 2007. Salvador, BA. Program and book of abstracts... São Carlos: Polymer Processing & Reology Center DEMa/UFSCar, 2007. Oral 062. p. 64.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
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| 19. | | MACÊDO, M. J. P. de; MATTOS, A. L. A.; COSTA, T. H. de C.; FEITOR, M. C.; ITO, E. N.; MELO, J. D. D. Effect of cold plasma treatment on recycled polyethylene/kapok composites interface adhesion. Composite Interfaces, Zeist, v. 26, n. 10, p. 871-886, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| 20. | | MARCONCINI, J. M.; ITO, E. N.; BRITTO, D. de; OLIVEIRA, R. M.; PAES, M. C. D.; TEIXEIRA, F. F.; ASSIS, 0. B. G. de. Metodologia de caracterização mecânica e termo-dinâmico-mecânica de palha de milho. In: WORKSHOP DA REDE DE NANOTECNOLOGIA APLICADA AO AGRONEGÓCIO, 3., 2007, Londrina. Anais... São Carlos: Embrapa Instrumentação Agropecuária, 2007. p. 106-108.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| Registros recuperados : 44 | |
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